Laboratoire de Microsondes Chromogenic in situ hybridization (CISH) – Microscope Electronique à Balayage
Comparative Study of Extra Capsular Cataract Extraction (ECCE) and Small Incision Cataract Surgery (SICS): Experience on Cataract Surgery in a Tertiary Center of Army Hospital, Kathmandu.
Cataract is a common disease of old age impede daily activities that lead to a poor quality of life due to poor vision. This is the most common cause of blindness are treatable. In practice, extracapsular cataract excision and incision cataract surgery is a small two-common surgery for cataract today in developing parts such as in Nepal.
Between these two cataract operations, ECCE and SICS; SICS provide better visual outcome.This retrospective cohort study conducted included 286 eyes operated either by conventional extracapsular cataract extraction or small incision cataract surgery. patient demographics, preoperative vision, postoperative parameters / variables studied from patient registration. Postoperative visual outcome and complications compared in both groups in the postoperative period and at 8 weeks follow up period.Among total of 286 cases, 138 and 148 ECCE undergo SICS undergo. 145 (50.6%) is the right eye, 141 (49.3%) is the left eye.
Among the number of operated cases, 123 (43%) are mature cataract, 97 (33.9%) of immature cataract, 66 (23.1%), hyper-mature cataracts. In 172 cases (60.1%) there is a good vision, in 104 cases (36.4%) was the limit while the remaining 10 (3.5%) cases was poor on postoperative day 60th. There is a better visual results (≥6 / 18) by SICS (78.3%) compared to ECCE (40.5%) (p <0.001) at 60 days post-surgery. Among the cases SICS undergo fewer complications than ECCE. (P <0.001, OR = 5.72 (2.10 to 15.51)). This study supports that SICS safer than ECCE with less complication rates and better visual outcomes in the short-term observation.
Build and explore the physical user interface requires high technical skills and special working hours. The behavior of some devices with heterogeneous channel input / output and connectivity must be programmed in a context where not only the software interface issues, but also the essential hardware components (for example, sensors and actuators).
Physical interaction prototyping hindered by challenges: (1) the interaction between the physical programming of sensors / actuators and digital interface; (2) perform the functions for the different platforms in different programming languages; and (3) build electronic objects custom-inserted.
Comparative Study of Extra Capsular Cataract Extraction (ECCE) and Small Incision Cataract Surgery (SICS): Experience on Cataract Surgery in a Tertiary Center of Army Hospital, Kathmandu.
Comparison of potential tolerance and local anti-inflammatory diclofenac and indomethacin eye drops in the postoperative management of patients undergoing ECCE with PCL.
Prostaglandins have been shown to mediate the various manifestations of inflammation of the eye. Diclofenac and indomethacin allegedly inhibit cyclooxygenase, an enzyme required for the biosynthesis of prostaglandins. Anti-inflammatory effects and subjective tolerance of topical diclofenac and indomethacin 0.1% 0.1% inspected in postoperative treatment of 64 patients who underwent extracapsular cataract surgery with IOL implantation in a prospective, comparative, single-masked study.
Patients with preexisting ocular disease or the course of intraoperative complicated excluded from the study. ophthalmological examinations performed prior to surgery and on the day: 1, 4-6, and 28-30 postoperatively. Tolerance was assessed by subjective patient evaluation.
Description: GFP or green fluorescents protein is a protein encoded by the GFP gene which is approximately 27 kDa. It functions as an energy-transfer acceptor by transducing the blue chemiluminescence of the protein aequorin into green fluorescent light via energy transfer. It fluoresces in vivo upon receiving energy from the Ca2+ activated photoprotein aequorin. Fluorescent proteins have become a useful tool for making chimeric proteins, where they function as a fluorescent protein tag. GFP is expressed specifically in photocytes. STJ97030 was developed from a plant specific clone and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects GFP, EGFP and GFP, EGFP tag fusion proteins in plants.
Description: Affinity purified Anti-GFP (Green Fluorescent Protein) Tag clone GF28R, mouse IgG1. Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP. Applications: Western Blot(1:1000-3000), Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.
Description: A polyclonal antibody against GFP. Recognizes GFP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;ELISA:1:2000-1:10000, WB:1:1000-1:10000
Description: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with asecondary reagent (such as dyes or antibodies) for detection.
Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.
Description: A polyclonal antibody against GFP-Tag. Recognizes GFP-Tag from . This antibody is Unconjugated. Tested in the following application: WB;WB:1:5000
Description: A monoclonal antibody for detection of GFP from Null. This GFP antibody is for WB, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP from Null. This GFP antibody is for WB, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP from Null. This GFP antibody is for WB, IP. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is unconjugated.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 565.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 633.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 655.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 680.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 700.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Alkaline Phosphatase.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to APC .
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to APC/Cy7.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Biotin.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Dylight 350.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Dylight 405.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Dylight 488.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Dylight 594.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Dylight 633.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to FITC.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to HRP.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to PE/ATTO 594.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to PerCP.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to RPE .
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Streptavidin.
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A monoclonal antibody for detection of GFP-Tag from Null. This GFP-Tag antibody is for WB. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen recombinant protein
Description: A polyclonal antibody against GFP Mouse Polyclonal. Recognizes GFP Mouse Polyclonal from N/A. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.IF:1/200-1/1000.ELISA:1/20000
Description: A polyclonal antibody against GFP Mouse Polyclonal. Recognizes GFP Mouse Polyclonal from N/A. This antibody is Unconjugated. Tested in the following application: WB, IHC, IP, IF, ELISA;WB:1/500-1/2000.IHC:1/100-1/300.IP:2-5ug/mglysate.IF:1/200-1/1000.ELISA:1/20000
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogen and conjugated to HRP. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogen and conjugated to HRP. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A polyclonal antibody for detection of GFP Tag from Null. This GFP Tag antibody is for WB. It is affinity-purified from rabbit antiserum by affinity-chromatography using the specific immunogen and conjugated to HRP. The antibody is produced in rabbit by using as an immunogen recombinant protein
Description: A Monoclonal antibody against Human GFP. The antibodies are raised in Mouse and are from clone 4B10B2. This antibody is applicable in WB and IHC, FC, E
There is no significant difference (p <0.05) in the postoperative inflammation between the two treatment groups. There is a tendency for more severe corneal edema at day 4-6 in the indomethacin group. Diclofenac was slightly better tolerated.
