Laboratoire de Microsondes Chromogenic in situ hybridization (CISH) – Microscope Electronique à Balayage
Comparative Study of Extra Capsular Cataract Extraction (ECCE) and Small Incision Cataract Surgery (SICS): Experience on Cataract Surgery in a Tertiary Center of Army Hospital, Kathmandu.
Cataract is a common disease of old age impede daily activities that lead to a poor quality of life due to poor vision. This is the most common cause of blindness are treatable. In practice, extracapsular cataract excision and incision cataract surgery is a small two-common surgery for cataract today in developing parts such as in Nepal.
Between these two cataract operations, ECCE and SICS; SICS provide better visual outcome.This retrospective cohort study conducted included 286 eyes operated either by conventional extracapsular cataract extraction or small incision cataract surgery. patient demographics, preoperative vision, postoperative parameters / variables studied from patient registration. Postoperative visual outcome and complications compared in both groups in the postoperative period and at 8 weeks follow up period.Among total of 286 cases, 138 and 148 ECCE undergo SICS undergo. 145 (50.6%) is the right eye, 141 (49.3%) is the left eye.
Among the number of operated cases, 123 (43%) are mature cataract, 97 (33.9%) of immature cataract, 66 (23.1%), hyper-mature cataracts. In 172 cases (60.1%) there is a good vision, in 104 cases (36.4%) was the limit while the remaining 10 (3.5%) cases was poor on postoperative day 60th. There is a better visual results (≥6 / 18) by SICS (78.3%) compared to ECCE (40.5%) (p <0.001) at 60 days post-surgery. Among the cases SICS undergo fewer complications than ECCE. (P <0.001, OR = 5.72 (2.10 to 15.51)). This study supports that SICS safer than ECCE with less complication rates and better visual outcomes in the short-term observation.
Build and explore the physical user interface requires high technical skills and special working hours. The behavior of some devices with heterogeneous channel input / output and connectivity must be programmed in a context where not only the software interface issues, but also the essential hardware components (for example, sensors and actuators).
Physical interaction prototyping hindered by challenges: (1) the interaction between the physical programming of sensors / actuators and digital interface; (2) perform the functions for the different platforms in different programming languages; and (3) build electronic objects custom-inserted.
Comparative Study of Extra Capsular Cataract Extraction (ECCE) and Small Incision Cataract Surgery (SICS): Experience on Cataract Surgery in a Tertiary Center of Army Hospital, Kathmandu.
Comparison of potential tolerance and local anti-inflammatory diclofenac and indomethacin eye drops in the postoperative management of patients undergoing ECCE with PCL.
Prostaglandins have been shown to mediate the various manifestations of inflammation of the eye. Diclofenac and indomethacin allegedly inhibit cyclooxygenase, an enzyme required for the biosynthesis of prostaglandins. Anti-inflammatory effects and subjective tolerance of topical diclofenac and indomethacin 0.1% 0.1% inspected in postoperative treatment of 64 patients who underwent extracapsular cataract surgery with IOL implantation in a prospective, comparative, single-masked study.
Patients with preexisting ocular disease or the course of intraoperative complicated excluded from the study. ophthalmological examinations performed prior to surgery and on the day: 1, 4-6, and 28-30 postoperatively. Tolerance was assessed by subjective patient evaluation.
Description: Goat polyclonal antibody to GFP (green fluorescent protein). GFP is a protein composed of 238 amino acid residues (26.9 kDa) that exhibits bright green fluorescence when exposed to blue light. In cell and molecular biology, the GFP protein is frequently used as a reporter of expression.
Description: Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Description: GFP or green fluorescents protein is a protein encoded by the GFP gene which is approximately 27 kDa. It functions as an energy-transfer acceptor by transducing the blue chemiluminescence of the protein aequorin into green fluorescent light via energy transfer. It fluoresces in vivo upon receiving energy from the Ca2+ activated photoprotein aequorin. Fluorescent proteins have become a useful tool for making chimeric proteins, where they function as a fluorescent protein tag. GFP is expressed specifically in photocytes. STJ97030 was developed from a plant specific clone and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects GFP, EGFP and GFP, EGFP tag fusion proteins in plants.
Description: The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Description: Affinity purified Anti-GFP (Green Fluorescent Protein) Tag clone GF28R, mouse IgG1. Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP. Applications: Western Blot(1:1000-3000), Dot Blot, ELISA, Immunocytochemistry(1:500-2000), Immunoprecipitation. Optimal dilutions/concentrations should be determined by the end user.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is unconjugated.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 390.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 488.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to ATTO 594.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to Biotin.
Description: A polyclonal antibody for GFP from Species Independent. The antibody is produced in Mouse after immunization with Full-length GFP protein.. The Antibody is tested and validated for WB, IHC, ICC/IF, IP assays with the following recommended dilutions: WB (1:5000), IHC (1:200), ICC/IF (1:200), IP (1:100). This GFP antibody is conjugated to FITC.
Description: Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Description: Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
Description: The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Description: The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
Description: Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
There is no significant difference (p <0.05) in the postoperative inflammation between the two treatment groups. There is a tendency for more severe corneal edema at day 4-6 in the indomethacin group. Diclofenac was slightly better tolerated.
