Manual small incision cataract surgery (MSICS) with posterior chamber intraocular lens versus extracapsular cataract extraction (ECCE) with posterior chamber intraocular lens for age-related cataract.

Manual small incision cataract surgery (MSICS) with posterior chamber intraocular lens versus extracapsular cataract extraction (ECCE) with posterior chamber intraocular lens for age-related cataract.

BACKGROUND
Age -related cataract is a clouding of the lens, which occurs as a result of lens protein denaturation. Age -related cataract remains the leading cause of blindness globally, except in the most advanced countries. A key question is what is the best way of eliminating the lens, especially in low-income settings.


OBJECTIVE
To compare two different techniques of elimination of the lens in cataract surgery: the user is operating a small incision (MSICS) and extracapsular cataract extraction (ECCE).


METHOD
We searched CENTRAL (which contains the Cochrane Eyes and Trials Vision Group Register) (2014, Issue 8), Ovid MEDLINE, Ovid MEDLINE in process and Non-Indexed Citations, Ovid MEDLINE Daily, Ovid OLDMEDLINE (January 1946-September 2014), EMBASE ( January 1980 until September 2014), Latin America and the Caribbean Health Sciences Literature database (LILACS) (January 1982 to September 2014), web of Science Conference Proceedings Citation Index, Science (CPCI-S), (January 1990 to September 2014), which metaRegister of Controlled Trials (mRCT) ), and the World Health Organization (WHO) International Clinical Trials Registry platform (ICTRP) . We do not use date or language restrictions in the electronic searches for trials. Last we are looking for an electronic database on 23 September 2014.


METHOD
We included randomized controlled trials (RCTs) only. Participants in the trials were people with age-related cataract. We included trials in which MSICS with posterior chamber intraocular lens (IOL) implant compared to ECCE with posterior chamber IOL implant.


METHOD
Data were collected independently by two authors. We aim to collect data on presenting visual acuity of 6/12 or better and best corrected visual acuity less than 6/60 in three months and one year after surgery. Other results included intraoperative complications, complications of long-term (one year or more after surgery), quality of life, and cost effectiveness. There is not enough data available from the trial and to conduct a meta-analysis.

 Manual small incision cataract surgery (MSICS) with posterior chamber intraocular lens versus extracapsular cataract extraction (ECCE) with posterior chamber intraocular lens for age-related cataract.
Manual small incision cataract surgery (MSICS) with posterior chamber intraocular lens versus extracapsular cataract extraction (ECCE) with posterior chamber intraocular lens for age-related cataract.


RESULTS
Three randomized trial allocate people with age-related cataract to MSICS or ECCE included in this review (n = 953 participants). Two trials conducted in India and one in Nepal. experimental methods, such as random allocation and allocation concealment, not clearly explained; only in one experiment was an attempt to conceal the outcome assessors. Three studies reported follow-up six to eight weeks after surgery. In two studies, a participant in the group MSICS achieved without the aid of visual acuity 6/12 or 6/18 or better than the ECCE group, but overall no more than 50% of people achieve a good functional vision in two studies.

10/806 (1.2%) of those enrolled in the two trials had poor outcomes after surgery (best corrected vision of less than 6/60) with no evidence of a difference in risk between the two techniques (risk ratio (RR) 1.58 , 95% confidence interval (CI) 0.45 to 5.55). Surgically induced astigmatism more common with ECCE procedure of MSICS in two trials that reported this outcome. In one study there more complications intra and post-surgery in MSICS group. One study reported that the cost of two similar procedures.

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bcl-2(100/D5) Antibody

BNC610045-100 100uL
EUR 238.8
Description: Primary antibody against bcl-2(100/D5), CF660R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC610045-500 500uL
EUR 652.8
Description: Primary antibody against bcl-2(100/D5), CF660R conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC680045-100 100uL
EUR 238.8
Description: Primary antibody against bcl-2(100/D5), CF568 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC680045-500 500uL
EUR 652.8
Description: Primary antibody against bcl-2(100/D5), CF568 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC470045-100 100uL
EUR 238.8
Description: Primary antibody against bcl-2(100/D5), CF647 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC470045-500 500uL
EUR 652.8
Description: Primary antibody against bcl-2(100/D5), CF647 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC700045-100 100uL
EUR 238.8
Description: Primary antibody against bcl-2(100/D5), CF770 conjugate, Concentration: 0.1mg/mL

bcl-2(100/D5) Antibody

BNC700045-500 500uL
EUR 652.8
Description: Primary antibody against bcl-2(100/D5), CF770 conjugate, Concentration: 0.1mg/mL

c-Jun (pS73 / 100) Antibody

20-abx133200
  • EUR 376.80
  • EUR 560.40
  • EUR 243.60
  • 100 ul
  • 200 ul
  • 30 ul

c-Jun (pS73/100) Antibody

E38PA4698 100ul
EUR 225
Description: Available in various conjugation types.

JUN / JUND (pS73 / 100) Antibody

20-abx328190
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

FAM azide, 5-isomer, 100 uL, 10 mM/DMSO

14130 100 ul
EUR 90

R6G azide, 5-isomer, 100 uL, 10 mM/DMSO

14430 100 µl
EUR 129.6

FAM azide, 6-isomer, 100 uL, 10 mM/DMSO

15130 100 ul
EUR 90

TAMRA azide, 5-isomer, 100 uL, 10 mM/DMSO

17130 100 µl
EUR 129.6

ROX azide, 5- isomer, 100 uL, 10 mM/DMSO

11230 100 µl
EUR 129.6

Medfuture Variable Volume Single Channel Pipette *100-1000 ul*

L55008-Each Each
EUR 109
Description: This single-channel pipette is easy to use.

Single channel mini-pipette (130 mm) 100 ul, autoclavable

SCMP-100 1
EUR 110.4

Protein Transport Inhibitor Cocktail (500X) 4 x 100 uL

E16FXP050-4x100 4x100 μl
EUR 323.17
Description: Available in various conjugation types.

Anti-S-100 beta antibody

STJ98374 100 µl
EUR 280.8
Description: Mouse monoclonal to S-100 beta.

s-100 Antibody (IHC Gold)

E36PA100 100ul
EUR 225
Description: Available in various conjugation types.

Anti-S-100 alpha antibody

STJ97685 200 µl
EUR 236.4
Description: Rabbit polyclonal to S-100 alpha.

Anti-S-100 alpha antibody

STJ98373 100 µl
EUR 280.8
Description: Mouse monoclonal to S-100 alpha.

Anti-S-100 alpha antibody

STJ95562 200 µl
EUR 236.4
Description: Rabbit polyclonal to S-100 alpha.

Anti-S-100 Protein antibody

STJ16101355 1 mL
EUR 717.6

GFP (FL) Antibody

48297-100ul 100ul
EUR 399.6

GFP (FL) Antibody

48297-50ul 50ul
EUR 286.8

GFP antibody (HRP)

60R-GG002hrp 1 mg
EUR 988.8
Description: Goat polyclonal GFP antibody (HRP) conjugated

GFP antibody (HRP)

61R-G103aHRP 1 mg
EUR 1389.6
Description: Mouse monoclonal GFP antibody (HRP)

GFP Antibody (YFP)

F52058-0.05ML 0.05 ml
EUR 140.25
Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

GFP Antibody / YFP

F52058-0.2ML 0.2 ml
EUR 330.65
Description: Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Human S-100 Protein Antibody

11561-05011 150 ug
EUR 260.4

S-100 α Polyclonal Antibody

E20-74197 100ug
EUR 225
Description: Available in various conjugation types.


CONCLUSION
No other studies from other countries besides India and Nepal and there are insufficient data cost-effectiveness of each procedure. Better evidence is needed before any changes can be implemented. Future studies should have a long-term follow-up and conducted to minimize bias revealed in this review with a larger sample size to allow inspection of its side effects.

Benoit

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