Objective: To discuss the efficacy and safety of two-incision extracapsular cataract extraction (ECCE) in the treatment of hard-core cataract with corneal endothelial cell density is low. Methods: This study is a retrospective series of case studies. Forty-six patients (47 eyes) with hard-core cataract and corneal endothelial cell density are enrolled in Shandong Eye Hospital from June 2009 to December 2018, including 22 men and 24 women, aged 50 to 74 (63.8 ± 6, 3 years. Preoperative corneal endothelial cell density of less than 1000 cells / mm (2), and cataract nuclear force is equal to or greater than the fourth grade.

According to the method of surgery, patients were divided into single-incision ECCE group (24 eyes) and group ECCE two incisions (23 eyes). Surgical procedures for double-incision group is as follows. First, the pre-tops scleral incision is made. Then the conventional capsulorhexis is done through a transparent 2.6 mm corneal tunnel incision in the temporal or nasal side, after the hydro-dissection is done.

Next, the surgeon cuts a pre-incision in the sclera, lens nucleus delivered, sutured scleral incision and removed the residual cortical material from corneal incision. Finally, foldable intraocular lens implantation, and viscoelastic substance has been removed. The intraoperative conditions and postoperative anterior chamber corneal edema condition monitored. During 6 months of follow-up after surgery, endothelial cell density, visual acuity and astigmatism in the two groups were compared. The χ (2) test was used to compare the data calculation, and t test was used to compare measurement data.

Results: No difference was significant (t = P = group ECCE, which is (827 ± 164) cells / mm (2) and (802 ± 121) cells / mm (2), respectively At 6 months after surgery ,. in two incisions and single-incision group, the density of endothelial cells is (793 ± 147) cells / mm (2) and (706 ± 101) cells / mm (2), respectively, and the difference was statistically significant (t = P

Conclusion: The double-incision ECCE, in which the core is delivered through an incision scleral lenses and other procedures done through a corneal tunnel incision, safe and effective for patients of cataract with hard core and low corneal endothelial cell density

Endogenous structure ESX-3 secretion system.

ESX secretion system (or Type VII) is a protein export system in mycobacteria and many Gram-positive bacteria that mediate a variety of functions including virulence, conjugation, and regulation of metabolism.

This system translocate folded dimer WXG100-superfamily protein substrates across the cytoplasmic membrane. We report cryo-electron microscopy structure of the system of ESX-3, purified using epitope tag recombineering inserted in the chromosomes of a model organism Mycobacterium smegmatis.

Vimentin
AP88730	SAB	1mg	EUR 2640

Vimentin
AP88734	SAB	1mg	EUR 2640

Vimentin
AP88803	SAB	1mg	EUR 2640

Vimentin
AP89050	SAB	1mg	EUR 2640

Vimentin
AP89299	SAB	1mg	EUR 2640

Vimentin
AP78769	SAB	1mg	EUR 2640

Vimentin
ant-245	ProSpec Tany	0.5mg	EUR 275
Description: Mouse Anti Human Vimentin

Vimentin
CH22108	Neuromics	100 ul	EUR 272.7

Vimentin
CH23010	Neuromics	100 ul	EUR 214.8

Vimentin
GWB-527FF1	GenWay Biotech	0.01 mg	Ask for price

Vimentin
GT22103	Neuromics	100 ul	EUR 292.5

Vimentin
E8ET1610-39	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

Vimentin
E8M1412-1	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

Vimentin
E8R1308-6	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

Vimentin
MBS555398-01mL	MyBiosource	0.1mL	EUR 410

Vimentin
MBS555398-5x01mL	MyBiosource	5x0.1mL	EUR 1705

Vimentin
MBS555893-01mL	MyBiosource	0.1mL	EUR 435

Vimentin
MBS555893-5x01mL	MyBiosource	5x0.1mL	EUR 1815

Vimentin
MBS555941-01mg	MyBiosource	0.1mg	EUR 435

Vimentin
MBS555941-5x01mg	MyBiosource	5x0.1mg	EUR 1815

Vimentin
MBS556404-01mL	MyBiosource	0.1mL	EUR 410

Vimentin
MBS556404-5x01mL	MyBiosource	5x0.1mL	EUR 1705

Vimentin
MBS370116-01mLConcentrate	MyBiosource	0.1mL(Concentrate)	EUR 225

Vimentin
MBS370116-05mLConcentrate	MyBiosource	0.5mL(Concentrate)	EUR 375

Vimentin
MBS370116-15mLRTU	MyBiosource	15mL(RTU)	EUR 425

Vimentin
MBS370116-3mLRTU	MyBiosource	3mL(RTU)	EUR 215

Vimentin
MBS370116-7mLRTU	MyBiosource	7mL(RTU)	EUR 270

Vimentin
MO22115	Neuromics	100 ul	EUR 272.7

Vimentin
MO22179	Neuromics	100 ug	EUR 292.5

Vimentin
MBS8533443-01mL	MyBiosource	0.1mL	EUR 305

Vimentin
MBS8533443-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

Vimentin
MBS8533443-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

Vimentin
MBS8533443-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

Vimentin
MBS8533443-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Vimentin
MBS8533952-01mL	MyBiosource	0.1mL	EUR 345

Vimentin
MBS8533952-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

Vimentin
MBS8533952-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

Vimentin
MBS8533952-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

Vimentin
MBS8533952-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Vimentin
MBS8534585-01mL	MyBiosource	0.1mL	EUR 345

Vimentin
MBS8534585-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

Vimentin
MBS8534585-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

Vimentin
MBS8534585-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

Vimentin
MBS8534585-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Vimentin
MBS8534714-01mL	MyBiosource	0.1mL	EUR 345

Vimentin
MBS8534714-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

Vimentin
MBS8534714-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

Vimentin
MBS8534714-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

Vimentin
MBS8534714-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565

Vimentin
MC-891	Kamiya Biomedical Company	VI-RE/1	Ask for price

Vimentin
MC-892	Kamiya Biomedical Company	V9	Ask for price

Vimentin
RA22124	Neuromics	100 ul of Serum	EUR 272.7

Vimentin
PR27081	Neuromics	20 ug	EUR 97.2

Vimentin
PR27081-B	Neuromics	each	EUR 184.5

Vimentin
pro-309	ProSpec Tany	20µg	EUR 60
Description: Recombinant Human Vimentin

Vimentin
V1016	Antagene	0.2mg	EUR 180

Vimentin (VIM)
MBS6508456-002mL	MyBiosource	0.02mL	EUR 435

Vimentin (VIM)
MBS6508456-01mL	MyBiosource	0.1mL	EUR 715

Vimentin (VIM)
MBS6508456-5x01mL	MyBiosource	5x0.1mL	EUR 3070

Vimentin FITC
ant-247	ProSpec Tany	0.5mg	EUR 350
Description: Mouse Anti Human Vimentin FITC

Vimentin, Human
LF-P0388	Abfrontier	0.5 mg	EUR 363.6
Description: Vimentin, Human protein

Human Vimentin
hAP-0952	Angio Proteomie	50ug	EUR 400

Vimentin ELISA
MBS494838-5x96Tests	MyBiosource	5x96Tests	EUR 3110

Vimentin ELISA
MBS494838-96Tests	MyBiosource	96Tests	EUR 680

Human Vimentin
MBS691558-002mg	MyBiosource	0.02mg	EUR 300

Human Vimentin
MBS691558-01mg	MyBiosource	0.1mg	EUR 450

Human Vimentin
MBS691558-5x01mg	MyBiosource	5x0.1mg	EUR 1725

Bovine Vimentin
7-06862	CHI Scientific	2µg	Ask for price

Bovine Vimentin
7-06863	CHI Scientific	10µg	Ask for price

Bovine Vimentin
7-06864	CHI Scientific	1mg	Ask for price

Vimentin Biotin
ant-246	ProSpec Tany	0.5mg	EUR 350
Description: Mouse Anti Human Vimentin Biotinylated

Bovine Vimentin
MBS143077-0002mg	MyBiosource	0.002mg	EUR 240

Bovine Vimentin
MBS143077-001mg	MyBiosource	0.01mg	EUR 310

Bovine Vimentin
MBS143077-1mg	MyBiosource	1mg	EUR 3770

Bovine Vimentin
MBS143077-5x1mg	MyBiosource	5x1mg	EUR 16645

Bovine Vimentin
rAP-5064	Angio Proteomie	Inquiry	Ask for price

Vimentin Protein
20-abx260337	Abbexa	Ask for price Ask for price Ask for price	20 ug 100 ug 1 mg

Vimentin Protein
abx260337-20g	Abbexa	20 µg	EUR 325

Vimentin Protein
abx260337-5g	Abbexa	5 µg	EUR 225

Vimentin Protein
20-abx263566	Abbexa	Ask for price Ask for price Ask for price	2 µg 5 ug 10 ug

Vimentin Peptide
43-649P	ProSci	0.1 mg	EUR 405.6

Vimentin protein
30R-2114	Fitzgerald	250 ug	EUR 1228
Description: Purified native Bovine Vimentin protein

Vimentin protein
30R-2134	Fitzgerald	100 ug	EUR 755
Description: Purified native Bovine Vimentin Protein

Vimentin protein
30R-2137	Fitzgerald	100 ug	EUR 649
Description: Purified recombinant Human Vimentin protein

Vimentin protein
30R-AV009	Fitzgerald	100 ug	EUR 1539
Description: Purified recombinant Human Vimentin protein

Vimentin protein
MBS536597-025mg	MyBiosource	0.25mg	EUR 1920

Vimentin protein
MBS536597-5x025mg	MyBiosource	5x0.25mg	EUR 8495

Vimentin protein
MBS536607-01mg	MyBiosource	0.1mg	EUR 950

Vimentin protein
MBS536607-5x01mg	MyBiosource	5x0.1mg	EUR 4115

Vimentin protein
MBS537604-01mg	MyBiosource	0.1mg	EUR 920

Vimentin protein
MBS537604-025mg	MyBiosource	0.25mg	EUR 1850

Vimentin protein
MBS537604-5x025mg	MyBiosource	5x0.25mg	EUR 8175

Vimentin protein
MBS836953-01mg	MyBiosource	0.1mg	EUR 1045

Vimentin protein
MBS836953-5x01mg	MyBiosource	5x0.1mg	EUR 4560

Vimentin antibody
10R-10357	Fitzgerald	100 ug	EUR 451
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-1903	Fitzgerald	100 ul	EUR 468
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-2081	Fitzgerald	100 ul	EUR 503
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-2386	Fitzgerald	5 mL	EUR 306
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-2434	Fitzgerald	5 mL	EUR 468
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-7803	Fitzgerald	500 ug	EUR 678
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-7911	Fitzgerald	100 ug	EUR 519
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8124	Fitzgerald	100 ug	EUR 557
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8125	Fitzgerald	100 ug	EUR 626
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8126	Fitzgerald	100 ug	EUR 462
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8545	Fitzgerald	100 ul	EUR 471.6
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8546	Fitzgerald	100 ul	EUR 471.6
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-8547	Fitzgerald	100 ul	EUR 471.6
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
10R-V104a	Fitzgerald	50 ug	EUR 492
Description: Mouse monoclonal Vimentin antibody

Vimentin antibody
20C-CR2052G	Fitzgerald	250 ul	EUR 392
Description: Goat polyclonal Vimentin antibody

Vimentin antibody
20R-1397	Fitzgerald	10 mg	EUR 276
Description: Sheep polyclonal Vimentin antibody

Vimentin antibody
20R-1681	Fitzgerald	100 ug	EUR 807.6
Description: Rabbit polyclonal Vimentin antibody

Vimentin Antibody
21488	SAB	100ul	EUR 319

Vimentin Antibody
21488-100ul	SAB	100ul	EUR 302.4

Vimentin Antibody
21488-50ul	SAB	50ul	EUR 224.4

Vimentin antibody
20R-VP004	Fitzgerald	100 uL	EUR 565
Description: Guinea Pig polyclonal Vimentin antibody

Vimentin antibody
20R-2697	Fitzgerald	50 ug	EUR 269
Description: Rabbit polyclonal Vimentin antibody

Vimentin antibody
20R-2962	Fitzgerald	100 ul	EUR 471.6
Description: Rabbit polyclonal Vimentin antibody

Vimentin Antibody
8C0390	AAT Bioquest	50ug	EUR 368
Description: Vimentin Antibody

Vimentin Antibody
3634-100	Biovision	each	EUR 405.6

Vimentin Antibody
3634-30T	Biovision	each	EUR 175.2

Vimentin Antibody
48952	SAB	100ul	EUR 499

Vimentin Antibody
48952-100ul	SAB	100ul	EUR 399.6

Vimentin Antibody
48952-50ul	SAB	50ul	EUR 286.8

Vimentin antibody
70R-35997	Fitzgerald	100 ug	EUR 294
Description: Rabbit polyclonal Vimentin antibody

Vimentin antibody
70R-36156	Fitzgerald	100 ug	EUR 294
Description: Rabbit polyclonal Vimentin antibody

Vimentin antibody
70R-50546	Fitzgerald	100 ul	EUR 286
Description: Purified Polyclonal Vimentin antibody

Vimentin antibody
70R-50547	Fitzgerald	100 ul	EUR 242
Description: Purified Polyclonal Vimentin antibody

Vimentin antibody
70R-2952	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal Vimentin antibody raised against the C terminal of VIM

Vimentin antibody
70R-3030	Fitzgerald	50 ug	EUR 467
Description: Rabbit polyclonal Vimentin antibody raised against the N terminal of VIM

Vimentin antibody
70R-30831	Fitzgerald	100 ug	EUR 294
Description: Rabbit polyclonal Vimentin antibody

Vimentin Antibody
E18-6131-1	EnoGene	50μg/50μl	EUR 145
Description: Available in various conjugation types.

Vimentin Antibody
E18-6131-2	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

Vimentin Antibody
E18-7013-1	EnoGene	50μg/50μl	EUR 145
Description: Available in various conjugation types.

Vimentin Antibody
E18-7013-2	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

Vimentin Antibody
E18-0292-1	EnoGene	50μg/50μl	EUR 145
Description: Available in various conjugation types.

Vimentin Antibody
E18-0292-2	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

Vimentin Antibody
E11-0390C	EnoGene	100μg	EUR 225
Description: Available in various conjugation types.

Vimentin Antibody
E10-20118	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

VIMENTIN Antibody
E10-20301	EnoGene	100μg/100μl	EUR 225
Description: Available in various conjugation types.

Vimentin Antibody
E033541	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

Vimentin Antibody
E021488	EnoGene	100μg/100μl	EUR 255
Description: Available in various conjugation types.

Vimentin antibody
CAF50631-100ug	Biomatik Corporation	100ug	EUR 338

Vimentin antibody
70R-10611	Fitzgerald	500 ug	EUR 532
Description: Affinity purified sheep polyclonal Vimentin antibody

Vimentin antibody
70R-11961	Fitzgerald	100 ug	EUR 371
Description: Rabbit polyclonal Vimentin antibody

Vimentin Antibody
33541-100ul	SAB	100ul	EUR 302.4

Vimentin Antibody
33541-50ul	SAB	50ul	EUR 224.4

Vimentin Antibody
ABF0292	Lifescience Market	100 ug	EUR 525.6

Vimentin Antibody
ABF6131	Lifescience Market	100 ug	EUR 525.6

Vimentin Antibody
ABF7013	Lifescience Market	100 ug	EUR 525.6

Vimentin Antibody
AF0292	Affbiotech	200ul	EUR 420

Vimentin Antibody
AF0292-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
AF0292-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
BF0071	Affbiotech	200ul	EUR 540

Vimentin Antibody
BF0071-100ul	Affinity Biosciences	100ul	EUR 210
Description: WB,IHC,ELISA

Vimentin Antibody
BF0071-200ul	Affinity Biosciences	200ul	Ask for price
Description: WB,IHC,ELISA

Vimentin Antibody
BF0071-50ul	Affinity Biosciences	50ul	EUR 150
Description: WB,IHC,ELISA

Vimentin PAT5H8AT
ant-682	ProSpec Tany	5µg	EUR 60
Description: Mouse Anti Human Vimentin AT5H8

Vimentin Antibody
B1242-100ul	Assay Biotech	100μl	EUR 217
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
B1242-50ul	Assay Biotech	50μl	EUR 143.5
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
B1243-100ul	Assay Biotech	100μl	EUR 217
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
B1243-50ul	Assay Biotech	50μl	EUR 143.5
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
C30875-100ul	Assay Biotech	100μl	EUR 217
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
C30875-50ul	Assay Biotech	50μl	EUR 143.5
Description: Vimentin Rabbit Polyclonal Antibody

Vimentin Antibody
AF7712	Affbiotech	200ul	EUR 540

Vimentin Antibody
AF7712-100ul	Affinity Biosciences	100ul	EUR 210
Description: WB,ELISA(peptide)

Vimentin Antibody
AF7712-200ul	Affinity Biosciences	200ul	EUR 270
Description: WB,ELISA(peptide)

Vimentin Antibody
AF7712-50ul	Affinity Biosciences	50ul	EUR 150
Description: WB,ELISA(peptide)

Vimentin Antibody
AF7013	Affbiotech	200ul	EUR 540

Vimentin Antibody
AF7013-100ul	Affinity Biosciences	100ul	EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
AF7013-200ul	Affinity Biosciences	200ul	EUR 270
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
AF7013-50ul	Affinity Biosciences	50ul	EUR 150
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
AF6131	Affbiotech	200ul	EUR 420

Vimentin Antibody
AF6131-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
AF6131-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
DF7035	Affbiotech	200ul	EUR 420

Vimentin Antibody
DF7035-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
DF7035-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IHC,IF/ICC,ELISA(peptide)

Vimentin Antibody
DF7052	Affbiotech	200ul	EUR 420

Vimentin Antibody
DF7052-100ul	Affinity Biosciences	100ul	EUR 168
Description: WB,IF/ICC,ELISA(peptide)

Vimentin Antibody
DF7052-200ul	Affinity Biosciences	200ul	EUR 210
Description: WB,IF/ICC,ELISA(peptide)

Vimentin Antibody
GWB-3589F0	GenWay Biotech	0.1 mg	Ask for price

Vimentin Antibody
GWB-703C97	GenWay Biotech	1 ml	Ask for price

Vimentin, Antibody
GWB-7259D7	GenWay Biotech	0.1 mg	Ask for price

Vimentin, Antibody
GWB-A7A1D3	GenWay Biotech	0.1 mg	Ask for price

The structure reveals stacked architecture that extends above and below the membrane in bacteria. ESX-3 protomer complex assembled from a single copy of EccB3, EccC3, and EccE3 and two copies of the proteins EccD3. In the structure, protomers form a stable dimer that is consistent with the assembling into larger oligomers. ESX-3 structure provides a framework for further study of this important bacterial transporter.