Blasticidin Selection

Description: GFP expression stable cells in A549 human lung cancer cell line with Blasticidin resistance

Description: Pre-made optional inducible lentiviral shRNA expression particles under human H1 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

Description: Media Catalog; Cell Culture Reagents

Description: Luciferase (firefry) stable cell line with Blasticidin marker.

Description: CRE recombinase stable cell line with Blasticidin resistance

Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

Description: GFP expression stable cells in RKO Cells, human colorectal carcinoma cells with Blasticidin resistance

Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Description: Premade lentivirus expressing human TERT gene under EF1a promoter. It does not contain any antibiotic selection.

Description: Luciferase (firefry) stable cell line with GFP-Blasticidin dual marker.

Description: Luciferase (firefry) stable cell line with RFP-Blasticidin dual marker.

Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.