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Hct116 Blasticidin

Lab Reagents

Human IgG antibody Laboratories manufactures the hct116 blasticidin reagents distributed by Genprice. The Hct116 Blasticidin reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Hct116 products are available in stock. Specificity: Hct116 Category: Blasticidin

Chemicals information

A549 / GFP stable cells (Blasticidin)

SC043-Bsd 2 x 106 cell/ml x 1ml
EUR 930
Description: GFP expression stable cells in A549 human lung cancer cell line with Blasticidin resistance

shRNA-U6 (Neg)-(blasticidin) lentivirus

U6(shRNA-Ctr)-Bsd 1 x107 IFU/ml x 200ul
EUR 418.8
Description: Pre-made lentiviral shRNA expression particles under human U6 promoter, containing a hairpin insert that should not knockdown any known human or mouse gene. This non-targeting control serves as a negative control for shRNA knockdown experiments. It also contains a Blasticidin marker under Rsv promoter.

Cas9-Expressing HCT116 Cell line – High expression

78135-H 2 vials
EUR 6500
Description: This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

Cas9-Expressing HCT116 Cell line – Low expression

78135-L 2 vials
EUR 6500
Description: This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limited dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a low-expressing cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

NF-κB Reporter Cellular Assay Pack (HCT116)

79326 2 vials
EUR 1245
Description: The NF-κB Reporter Cellular Assay Pack provides all the key reagents required to monitor the activity of the nuclear factor Kappa B (NF-κB) signal transduction pathways. The pack contains the NF-κB Reporter (Luc)- HCT-116 Recombinant Cell Line, a luciferase reporter cell line that contains a firefly luciferase gene under the control of four copies of the NF-κB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or stimulants of lymphokine receptors, endogenous NF-κB transcription factors bind to the DNA response elements, inducing transcription of the luciferase reporter gene. This cell line is validated for the response to TNFalpha and to treatment with NF-κB inhibitor, evodiamine._x000D_Additionally, the pack includes cell culture medium (Thaw Medium 7) that has been optimized for use with HCT-116 cells. Thaw Medium 7 includes 10% fetal bovine serum and 1% Pen/Strep. Finally, the pack provides the ONE-Step™ Luciferase Detection System. This reagent provides highly sensitive, stable detection of firefly (Photinus pyralis) luciferase activity. The ONE-Step luciferase reagent can be used directly in cells in growth medium, and can be detected with any luminometer; automated injectors are not required._x000D__x000D_

NF-κB Reporter (Luc) - HCT116 Cell Line

60623 2 vials
EUR 1095
Description: NF-B luciferase reporter construct is stably integrated into the genome of HCT-116 cells. The
firefly luciferase gene is controlled by 4 copies of NF-κB response element located upstream of
the TATA promoter. Following activation by stimulants, endogenous NF-κB transcription factors
bind to the DNA response elements to induce transcription of the luciferase gene._x000D_The NF-κB-luciferase/HCT-116 cell line is suitable for monitoring the activity of NF-κB signaling
in response to stimulants such as the cytokines TNF and IL-1β, pathogen-associated
molecular pattern (PAMP) (i.e. flagellin) or endogenous damage-associated molecular pattern
(DAMP) molecules (i.e. NOD1 ligand) (see application references). It is also suitable for
establishing cell-based screens for inhibitors that target specific NF-κB stimulating molecules.
This cell line can be further modified to allow investigation of downstream NF-κB activities as a
result of targeted genetic mutation(s).

luciferase (firefly), HEK293 stable cells (Blasticidin)

SC002-Bsd 2 x 106 cell/ml x 1ml
EUR 843.6
Description: Luciferase (firefry) stable cell line with Blasticidin marker.

CRE stable cells with Blasticidin marker

SC004-Bsd 2 x 106 cell/ml x 1ml
EUR 1365.6
Description: CRE recombinase stable cell line with Blasticidin resistance

Luciferase (firefly), Hela stable cells (Blasticidin)

SC032-Bsd 2 x 106 cell/ml x 1ml
EUR 1452
Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

RKO / GFP Stable Cell Line (Blasticidin)

SC047-GB 2 x 106 cell/ml x 1ml
EUR 1452
Description: GFP expression stable cells in RKO Cells, human colorectal carcinoma cells with Blasticidin resistance

pSMPUW-IRES-Blasticidin Lentiviral Expression Vector

VPK-219 10 µg
EUR 748.8
Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

EZSolution? Blasticidin S Hydrochloride, Sterile-Filtered

2805-10 each
EUR 588

Myc Reporter (Luc) - HCT116 Cell Line (Myc Signaling Pathway)

60520 2 vials
EUR 2175
Description: The Myc Reporter - HCT116 cell line contains the firefly luciferase gene under the control of Myc responsive elements stably integrated into HCT116 cells, a human colon cancer cell line. HCT116 contains a mutated beta-catenin which leads to the accumulation of β-catenin and constitutive activation of downstream Myc that induces the expression of Myc luciferase reporter. The cell line is validated for the inhibition of the expression of Myc luciferase reporter.

luciferase (firefly), HEK293 stable cells (GFP-Blasticidin)

SC002-GB 2 x 106 cell/ml x 1ml
EUR 1104
Description: Luciferase (firefry) stable cell line with GFP-Blasticidin dual marker.

luciferase (firefly), HEK293 stable cells (RFP, Blasticidin)

SC002-RB 2 x 106 cell/ml x 1ml
EUR 1104
Description: Luciferase (firefry) stable cell line with RFP-Blasticidin dual marker.

Luciferase-2A-GFP, Hela stable cells (Blasticidin)

SC032-GB 2 x 106 cell/ml x 1ml
EUR 2148
Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Blasticidin marker.

Luciferase-2A-RFP, Hela stable cells (Blasticidin)

SC032-RB 2 x 106 cell/ml x 1ml
EUR 2148
Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Blasticidin marker.

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