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Invivogen Puromycin

Puromycin

P097-100MG 100 mg
EUR 452.4

Human IgG antibody Laboratories manufactures the invivogen puromycin reagents distributed by Genprice. The Invivogen Puromycin reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact InVivoGen. Other Invivogen products are available in stock. Specificity: Invivogen Category: Puromycin

Puromycin

1mg Ask for price
Description: Puromycin

Puromycin

50mg Ask for price
Description: Puromycin

Puromycin

5mg Ask for price
Description: Puromycin

Puromycin

5 mg
EUR 71.53
Description: C22H29N7O5

Puromycin

50.0mg
EUR 450

Puromycin

1.0 ml; 10 mg/ml
EUR 60
Description: Extracted from Streptomyces alboniger bacteria, Puromycin is an aminonucleoside antibiotic that inhibits protein synthesis by interfering with the translation process. It specifically inhibits peptidyl transfer on both prokaryotic and eukaryotic ribosomes. The antibiotic inhibits the growth of Gram positive bacteria and various animal and insect cells. Fungi and Gram negative bacteria are resistant to Puromycin due to its low permeability. But in some particular conditions puromycin can be used for E. coli. For more than 30 years, puromycin has been widely used as a basic tool for studying protein synthesis. Now, puromycin hydrochloride is particularly useful for the selection of cell types harbouring plasmids carrying puromycin resistance genes. ;RESISTANCE TO PUROMYCIN;

The pac gene encoding a Puromycin N-acetyl-transferase (PAC) has been isolated from a Streptomyces productin strain1,2. It is located in a region of the pur cluster linked to the other genes determining the puromycin biosynthetic pathway. The expression of pac gene confers puromycin resistance to transfected mammalian cells3 expressing it. Under some conditions puromycin could also be used for selection of E. coli strains transformed with plasmids carrying the pac gene.

CONDITIONS OF SELECTION

Puromycin is poorly active on E. coli but is particularly useful in experiments conducted with mammalian cells. It can be used as an alternative to the Neomycin system for transfection experiments.

Mammalian cells

The working concentrations of puromycin for mammalian cell lines range from 1 to 10µg/mL. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach form the plates allowing easy and early identification of transformant clones.

References:

1) VARA J., et al. (1985). Biochemistry. 24: 8074-8081

2) LACALLE R.A., et al. (1989). Gene. 79: 375-380

3) DE LA LUNA S and J. ORTIN. (1992). Methods In Enzymology. 216: 376-385

JBS True Blue

300 µl
EUR 16
Description: JBS True Blue

Chemicals information

Puromycin Dihydrochloride

1860-500 each
EUR 966

Puromycin dihydrochloride

B7587-5.1 10 mM (in 1mL H2O)
EUR 142.8
Description: Puromycin dihydrochloride [1] is an analog of aminoacyl-tRNA.

Puromycin dihydrochloride

B7587-50 50 mg
EUR 157.2
Description: Puromycin dihydrochloride [1] is an analog of aminoacyl-tRNA.

Puromycin aminonucleoside

A3740-250 250 mg
EUR 1284
Description: IC50: N/APuromycin aminonucleoside, 3'-Amino-3'-deoxy-N6,N6-dimethyladenosine, is the aminonucleoside portion of the antibiotic puromycin. Puromycin aminonucleoside (PAN)-induced nephrosis in rats can provide a model for investigating the pathogenesis of severe proteinuric conditions.

Puromycin aminonucleoside

A3740-5.1 10 mM (in 1mL DMSO)
EUR 129.6
Description: IC50: N/APuromycin aminonucleoside, 3'-Amino-3'-deoxy-N6,N6-dimethyladenosine, is the aminonucleoside portion of the antibiotic puromycin. Puromycin aminonucleoside (PAN)-induced nephrosis in rats can provide a model for investigating the pathogenesis of severe proteinuric conditions.

Puromycin aminonucleoside

A3740-50 50 mg
EUR 366
Description: IC50: N/APuromycin aminonucleoside, 3'-Amino-3'-deoxy-N6,N6-dimethyladenosine, is the aminonucleoside portion of the antibiotic puromycin. Puromycin aminonucleoside (PAN)-induced nephrosis in rats can provide a model for investigating the pathogenesis of severe proteinuric conditions.

Puromycin Dihydrochloride

P001-1G 1 g
EUR 604.8

Puromycin Dihydrochloride

P001-250MG 250 mg
EUR 192

Puromycin Dihydrochloride

P001-500MG 500 mg
EUR 342

Puromycin Aminonucleoside

P041-100MG 100 mg
EUR 519.6

Puromycin Aminonucleoside

P041-1G 1g
EUR 2793.6

Puromycin Aminonucleoside

P041-500MG 500 mg
EUR 1636.8

Puromycin dihydrochloride

GA1940-100MG 100 mg
EUR 294

Puromycin dihydrochloride

GA1940-10MG 10 mg
EUR 84

Puromycin dihydrochloride

GA1940-250MG 250 mg
EUR 562.8

Puromycin dihydrochloride

GA1940-25MG 25 mg
EUR 132

Puromycin dihydrochloride

GA1940-500MG 500 mg
EUR 982.8

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