Thermofisher Blasticidin

Description: CRE recombinase stable cell line with Blasticidin resistance

Description: Luciferase (firefry) expression stable cell line in Hela cells with Blasticidin marker.

Description: GFP expression stable cells in RKO Cells, human colorectal carcinoma cells with Blasticidin resistance

Description: Clone your gene of interest into this Lentiviral Expression Vector, then co-transfect along with lentiviral packaging vectors into a packaging cell line such as 293LTV. This expression vector is compatible with any 2nd or 3rd generation lentiviral packaging system, but due to its design it is best matched with our ViraSafe packaging vectors to produce the highest viral titer.

Description: Luciferase (firefry) stable cell line with GFP-Blasticidin dual marker.

Description: Luciferase (firefry) stable cell line with RFP-Blasticidin dual marker.

Description: Luciferase (firefry) and GFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Luciferase (firefry) and RFP co-expression stable cell line in Hela cells with Blasticidin marker.

Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. Our Ecotropic Expression Systems assemble lentiviruses with an ecotropic envelope protein which will readily infect only mouse and rat cells via receptor-mediated binding, providing an additional level of safety. However, ecotropic viruses are not as stable upon freezing and will not survive ultracentrifugation procedures.

Description: Lentivirus based on HIV-1 provides a powerful means for gene expression because it can infect both dividing and non-dividing cells. Other 3rd generation lentiviral packaging systems provide a reduced risk of replication-competent virus, but a small risk remains. Our ViraSafe Lentivirus Expression Complete Systems provide a safer method even compared to other 3rd generation expression systems. Overlap with native HIV genes has been reduced much further, thereby substantially reducing the chance of generating replication-competent virus. The system has been engineered so that lentiviral titers remain at the levels of third generation systems. The Pantropic Expression Systems assemble VSVG-pseudotyped lentiviruses which can easily infect virtually any cells regardless of their species of origin.

Description: CRE recombinase stable cell line with Blasticidin resistance and RFP fluorescent marker.

Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1). AAV Helper Free Complete Expression Systems are available for native serotypes 1 through 6, as well as the novel AAV-DJ and AAV-DJ/8. The AAV-DJ system provides a hybrid capsid created by DNA shuffling technology combining 8 different native serotypes: AAV-2, AAV-4, AAV-5, AAV-8, AAV-9, avian AAV, bovine AAV, and caprine AAV. The result is a highly infectious vector that can transduce a wide variety of cells and tissues at significantly higher rates than AAV-2.

Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).

Description: The AAV Helper Free System produces recombinant AAV containing your gene of interest without the need to use a helper adenovirus. The adenoviral genes required for proper AAV packaging are provided in the pHelper plasmid (E2A, E4 and VA RNA) or in the 293 packaging cells (E1).